Rouille 0.1.6 through 3.6.2 contains a reachable assertion vulnerability in the Request::remove_prefix function that allows remote unauthenticated attackers to crash the server by sending a crafted percent-encoded URL. Attackers can send a request whose decoded path matches a configured prefix while the raw percent-encoded path does not, causing the assert! to fail and triggering either a 500 error or full process termination depending on the panic configuration.