A null byte (NUL character) can have different meanings across representations or languages. For example, it is a string terminator in standard C libraries, but Perl and PHP strings do not treat it as a terminator. When two representations are crossed - such as when Perl or PHP invokes underlying C functionality - this can produce an interaction error with unexpected results. Similar issues have been reported for ASP. Other interpreters written in C might also be affected.

The poison null byte is frequently useful in path traversal attacks by terminating hard-coded extensions that are added to a filename. It can play a role in regular expression processing in PHP.